Knowing the precise locations of nucleosomes in a genome is key to understanding how
genes are regulated. Recent 'next generation' ChIP–chip and ChIP–Seq technologies have …

Advances in genomics technology have provided the means to probe myriad chromatin
interactions at unprecedented spatial and temporal resolution. This has led to a profound …

Regulation of eukaryotic gene expression is far more complex than one might have imagined
30 years ago. However, progress towards understanding gene regulatory mechanisms …

In reconstituted reactions, Spl stimulates transcription at TATA-containing promoters in the
presence of semipurified TFIID fractions from either human or Drosophila cells, but is unable …

Comparative genomics of nucleosome positions provides a powerful means for understanding
how the organization of chromatin and the transcription machinery co-evolve. Here we …

The nucleosome is the fundamental building block of eukaryotic chromosomes. Access to
genetic information encoded in chromosomes is dependent on the position of nucleosomes …

Chromatin immunoprecipitation (ChIP-chip and ChIP-seq) assays identify where proteins
bind throughout a genome. However, DNA contamination and DNA fragmentation …

Most nucleosomes are well-organized at the 5′ ends of S. cerevisiae genes where “−1”
and “+1” nucleosomes bracket a nucleosome-free promoter region (NFR). How nucleosomal …

Despite being one of the first eukaryotic transcriptional regulatory elements identified, the
sequence of a native TATA box and its significance remain elusive. Applying criteria …

In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of
the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is …